Generator

Part:BBa_K1741008:Design

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)


sfGFP under promoter xylA1 with improved 5'UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 419


Design Notes

We have transplanted 5'UTR from the strong constitutive promoter proD.


Source

All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.

References